This article is part of the supplement: 51st Annual Meeting of the Society for Research into Hydrocephalus and Spina Bifida .

Oral presentation

Ultrastructural study of the permeability of in-vitro and ex-vivo human models of human arachnoid granulation CSF outflow pathway

Deborah M Grzybowski^1,2 , David W Holman¹ and Shelley A Glimcher¹

¹  Biomedical Engineering Department, The Ohio State University, Columbus, OH 43210, USA

²  Department of Ophthalmology, The Ohio State University, Columbus, OH 43210, USA

author email corresponding author email

from 51^st Annual Meeting of the Society for Research into Hydrocephalus and Spina Bifida
Heidelberg, Germany. 27–30 June 2007

Cerebrospinal Fluid Research 2007, 4(Suppl 1):S6doi:10.1186/1743-8454-4-S1-S6

Published:	20 December 2007

First paragraph (this article has no abstract)

In communicating hydrocephalus and also idiopathic intracranial hypertension, disturbed CSF dynamics may result from an increased resistance to CSF outflow at the arachnoid granulations (AGs). To better understand the mechanism of CSF egress, we modelled the outflow of CSF through human AGs using both cell culture (in-vitro) and whole tissue (ex-vivo) perfusion models. Ultrastructural studies were done using microparticles, ruthenium red, and TEM and to elucidate the mechanism of fluid flow.