Figure 2.

RT-PCR analysis of expression of junctional proteins in CP cell lines. A. Expression of TJ and AJ proteins in Z310 and TR-CSFB3 cells, two immortalized cell lines derived from rat CP epithelium. B. Expression of TJ and AJ proteins in the CPC-2 cell line derived from human CP carcinoma. The number of cycles of amplification is shown for each lane. Cldn 1, Cldn 2, Cldn 11, Ocln, and E-cdh are claudin 1, claudin 2, claudin 11, occludin, and E-cadherin, respectively. rCP and hCP are rat and human CP tissues used as a positive control.