Figure 3.

Capillary depletion analysis of [³H]PMPA and D-[¹⁴C]mannitol perfused cerebrum samples. Each point represents mean ± S.E.M., n = 3–6. There were no significant differences between the distribution of [³H]PMPA and D-[¹⁴C]mannitol in the whole brain homogenate, the brain parenchyma (supernatant) or the cerebral capillary endothelial cell enriched pellet at any time point. Accumulation in the pellet and supernatant fractions does not equal the uptake measured in the homogenate because all the data was standardized by weight. It is estimated that the cerebral capillary endothelial cell component only represents approximately 0.1% of the whole brain homogenate.